ABSTRACT
Objective To explore changes of invasion capability of cancer stem cells loading miR30a derived from enlarging and proliferative axillary lymph node of patients with breast cancer in nude mice.Methods MiR30a oligonucleotide fragment loaded by adenovirus vector was transfected into breast cancer stem cells isolated from enlarging and proliferative axillary lymphnodes of patients with breast cancer,MDA-MB-231 cell lines as the control.The cancer cells were injected into axillary subcutaneous fat of the nude mice for several weeks,and then,the expression of Vimentin or N-Cadherin in tumor tissues of each group of the mice was determined by immunohistochemistry and western blot.Results The average transfection rate of cancer stem cells loaded by miR30a was 62.5％,while it was 78.2％ for MDA-MB-231.The tumor volume was larger in adenovirus vector groups or in control groups of nude mice than in experimental groups induced by mir30a.Vimentin or N-cadherin in tumor tissues was significantly downexpressed in experimental groups with mir30a ((13.1±1.7)％,(15.3％±2.1)％)compared with that in adenovirus vector groups or in control groups((21.1±1.4)％,(25.3±1.6)％,P＜0.05),respectively.The difference between adenovirus vector groups and in control groups had no significant difference (P＞0.05).After inoculated for 6 weeks,except the subcutaneous plantations,no distant metastasis in nude mice was found.MDA-MB-231 cell lines groups had similar results.Conclusion The proliferative and invasive capability of cancer stem cells can be inhibited by miR30a,suggesting a new therapy for breast cancer.
ABSTRACT
Objectives To detect the expression level of miR-155 in tissues of colon cancer,and to investigate its influence on the occurrence,development and metastasis of colon cancer.Methods 69 cases with primary colon cancer treated with surgical resection in our hospital were chosen.Tissue samples of the tumor and tissue in the side of tumor(control)were collected and the expression level of miR-155 in the samples was detected by fluorescence quantitative polymerase chain reaction.The results were statically analyzed.Results The expression level of miR-155 was 0.471±0.100,0.238 ±0.108,0.719 ± 0.135 in three groups of colon cancer patients with tumor sizes ≥ 5 cm,high differentiation of tumor,and occurrence of lymphatic metastasis,respectively,and 0.623 ± 0.134,0.142 ±0.094,0.390±0.121 in their controls.And the level of miR-155 was 0.395±0.101 in patients with T1 + T2 tumor local infiltration,0.466±0.128 in T3 + T4 local infiltration,0.404±0.108 in Ⅰ + Ⅱ stage of TNM and 0.457±0.119 in Ⅲ± Ⅳ stage.The miR-155 was relatively higherly expressed in patients with tumor sizes≥5 cm,lower degree of differentiation,higher tumor infiltration degree,Ⅲ and Ⅳ stage of TNM,(t=3.002,12.531,20.425,2.576,1.908,P ＜ 0.05).Conclusions The expression level of miR-155 is correlated with tumor sizes,differentiation degrees,tumor infiltrating degrees,TNM and lymphatic metastasis,which could be used as diagnostic indexes for occurrence,development and metastasis of colon cancer.